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- Title
Secretion of human interferon- β 1b by recombinant Lactococcus lactis.
- Authors
Zhuang, Zhong; Wu, Zhi-gang; Chen, Min; Wang, Peng
- Abstract
Interferon- β has anti-viral, anti-proliferation and multifunctional immunomodulatory activities and shows promising clinical effects for treatment of inflammatory disorders. The recombinant human interferon- β (huIFN- β) 1b was expressed in the food-grade lactic acid bacterium, Lactococcus lactis, using a nisin-controlled gene expression system. huIFN- β production from recombinant strains (with and without LEISSTCDA propeptide) was approximately 21 and 7 μg l−1, respectively. Moreover, 95% (former strain) and 88% (latter strain) of total recombinant proteins were secreted into the culture medium. The biological activities of huIFN- β from recombinant strains revealed similar antiviral activities of 107 I.U. mg−1. These results demonstrate the potential application of recombinant strains as a food grade vehicle to deliver bioactive huIFN- β in vivo.
- Subjects
LACTOCOCCUS lactis; SECRETION; INTERFERONS; IMMUNOLOGICAL adjuvants; GENE expression; INFLAMMATION
- Publication
Biotechnology Letters, 2008, Vol 30, Issue 10, p1819
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-008-9761-y