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- Title
Asymmetric reduction of ethyl 4-chloro-3-oxobutanoate to ethyl (R)-4-chloro-3-hydroxybutanoate with two co-existing, recombinantEscherichia colistrains.
- Authors
Ying Liu; Zhinan Xu; Keju Jing; Xiaoxia Jiang; Jianping Lin; Fang Wang; Peilin Cen
- Abstract
Two recombinant strains,E. coliM15 (pQE30-alr0307) andE. coliM15 (pQE30-gdh0310), which were constructed to express, respectively, an NADPH-dependent aldehyde reductase gene and a glucose dehydrogenase gene, were mixed in an appropriate ratio and used for the asymmetric reduction of ethyl 4-chloro-3-oxobutanoate to ethyl (R)-4-chloro-3-hydroxybutanoate. The former strain acted as catalyst and the latter functioned in NADPH regeneration. The biotransformation was completed effectively without any addition of glucose dehydrogenase or NADP+/NADPH. An optical purity of 99% (ee) was obtained and the product yield reached 90.5% from 28.5 mMsubstrate.
- Subjects
ALCOHOL dehydrogenase; ESCHERICHIA coli; DEHYDROGENASES; ESCHERICHIA; ZINC enzymes; BIOENGINEERING; BIOCHEMISTRY
- Publication
Biotechnology Letters, 2005, Vol 27, Issue 2, p119
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-004-7336-0