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- Title
A physical, genetic and functional sequence assembly of the barley genome.
- Authors
Mayer, Klaus F. X.; Waugh, Robbie; Langridge, Peter; Close, Timothy J.; Wise, Roger P.; Graner, Andreas; Matsumoto, Takashi; Sato, Kazuhiro; Schulman, Alan; Muehlbauer, Gary J.; Stein, Nils; Ariyadasa, Ruvini; Schulte, Daniela; Poursarebani, Naser; Zhou, Ruonan; Steuernagel, Burkhard; Mascher, Martin; Scholz, Uwe; Shi, Bujun; Madishetty, Kavitha
- Abstract
Barley (Hordeum vulgare L.) is among the world's earliest domesticated and most important crop plants. It is diploid with a large haploid genome of 5.1 gigabases (Gb). Here we present an integrated and ordered physical, genetic and functional sequence resource that describes the barley gene-space in a structured whole-genome context. We developed a physical map of 4.98?Gb, with more than 3.90?Gb anchored to a high-resolution genetic map. Projecting a deep whole-genome shotgun assembly, complementary DNA and deep RNA sequence data onto this framework supports 79,379 transcript clusters, including 26,159 'high-confidence' genes with homology support from other plant genomes. Abundant alternative splicing, premature termination codons and novel transcriptionally active regions suggest that post-transcriptional processing forms an important regulatory layer. Survey sequences from diverse accessions reveal a landscape of extensive single-nucleotide variation. Our data provide a platform for both genome-assisted research and enabling contemporary crop improvement.
- Subjects
BARLEY genetics; NUCLEOTIDE sequence; PLANT genomes; RNA; GENOMES
- Publication
Nature, 2012, Vol 491, Issue 7426, p711
- ISSN
0028-0836
- Publication type
Article
- DOI
10.1038/nature11543