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- Title
Essential Amino Acid Supplementation by Gut Microbes of aWood-Feeding Cerambycid.
- Authors
Ayayee, Paul A.; Larsen, Thomas; Rosa, Cristina; Felton, Gary W.; Ferry, James G.; Hoover, Kelli
- Abstract
Insects are unable to synthesize essential amino acids (EAAs) de novo, thus rely on dietary or symbiotic sources for them. Wood is a poor resource of nitrogen in general, and EAAs in particular. In this study, we investigated whether gut microbiota of the Asian longhorned beetle, Anoplophora glabripennis (Motschulsky), a cerambycid that feeds in the heartwood of healthy host trees, serve as sources of EAAs to their host under different dietary conditions. δ13C-stable isotope analyses revealed significant δ13C-enrichment (3.460.1%; mean6SEM) across five EAAs in wood-fed larvae relative to their woody diet. δ13C values for the consumers greater than 1% indicate significant contributions from non-dietary EAA sources (symbionts in this case). In contrast, δ13C-enrichment of artificial diet-fed larvae (controls) relative to their food source was markedly less (1.7±0.1%) than was observed in wood-fed larvae, yet still exceeded the threshold of 1%. A predictive model based on δ13CEAA signatures of five EAAs from representative bacterial, fungal, and plant samples identified symbiotic bacteria and fungi as the likely supplementary sources of EAA in wood-fed larvae. Using the same model, but with an artificial diet as the dietary source, we identified minor supplementary bacterial sources of EAA in artificial diet-fed larvae. This study highlights how microbes associated with A. glabripennis can serve as a source of EAAs when fed on nutrient-limited diets, potentially circumventing the dietary limitations of feeding on woody substrates.
- Subjects
CERAMBYCIDAE; ESSENTIAL amino acids; GUT microbiome; HEARTWOOD; INSECT feeding &; feeds
- Publication
Environmental Entomology, 2016, Vol 45, Issue 1, p66
- ISSN
0046-225X
- Publication type
Article
- DOI
10.1093/ee/nvv153