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- Title
Human alveolar bone cell proliferation, expression of osteoblastic phenotype, and matrix mineralization on porous titanium produced by powder metallurgy.
- Authors
Rosa, Adalberto Luiz; Crippa, Grasiele Edilaine; De Oliveira, Paulo Tambasco; Taba Jr, Mario; Lefebvre, Louis‐Philippe; Beloti, Marcio Mateus
- Abstract
Objective: This study aimed at investigating the influence of the porous titanium (Ti) structure on the osteogenic cell behaviour. Materials and methods: Porous Ti discs were fabricated by the powder metallurgy process with the pore size typically between 50 and 400 μm and a porosity of 60%. Osteogenic cells obtained from human alveolar bone were cultured until subconfluence and subcultured on dense Ti (control) and porous Ti for periods of up to 17 days. Results: Cultures grown on porous Ti exhibited increased cell proliferation and total protein content, and lower levels of alkaline phosphatase (ALP) activity than on dense Ti. In general, gene expression of osteoblastic markers–runt-related transcription factor 2, collagen type I, alkaline phosphatase, bone morphogenetic protein-7, and osteocalcin was lower at day 7 and higher at day 17 in cultures grown on porous Ti compared with dense Ti, a finding consistent with the enhanced growth rate for such cultures. The amount of mineralized matrix was greater on porous Ti compared with the dense one. Conclusion: These results indicate that the porous Ti is an appropriate substrate for osteogenic cell adhesion, proliferation, and production of a mineralized matrix. Because of the three-dimensional environment it provides, porous Ti should be considered an advantageous substrate for promoting desirable implant surface–bone interactions.
- Subjects
CELL proliferation; BONE cells; EXTRACELLULAR matrix proteins; GENETIC regulation; GENE expression; PHENOTYPES
- Publication
Clinical Oral Implants Research, 2009, Vol 20, Issue 5, p472
- ISSN
0905-7161
- Publication type
Article
- DOI
10.1111/j.1600-0501.2008.01662.x