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- Title
A fast micromethod for the estimation of nisin activity in a soft cheese.
- Authors
Oros‐Flores, Zuleyka S; García‐Almendárez, Blanca E; Barboza‐Corona, José E; Salcedo‐Hernández, Rubén
- Abstract
The British Standard protocol currently used to determine the nisin concentration in cheese requires the manipulation of a 40 g sample involves two critical points, pH adjustment and heating/cooling. In this work, we developed a fast micromethod that permits the manipulation of 0.2 g cheese, substitutes the use of 0.02N HCl by 50 mM citric acid and facilitates the handling of many samples in reduced time, and with increased efficiency/yield. This change keeps the pH stable at ~3.4, enough to sidestep the pH adjustment, and nisin antimicrobial activity was stable at boiling temperatures.
- Subjects
NISIN; CHEESE; DAIRY products; LISTERIA monocytogenes; LACTOCOCCUS lactis
- Publication
International Journal of Dairy Technology, 2019, Vol 72, Issue 2, p282
- ISSN
1364-727X
- Publication type
Article
- DOI
10.1111/1471-0307.12583