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- Title
Lenzimycins A and B, Metabolites With Antibacterial Properties From Brevibacillus sp. Associated With the Dung Beetle Onthophagus lenzii.
- Authors
An, Joon Soo; Hong, Seong-Heon; Somers, Elisabeth; Lee, Jayho; Kim, Byung-Yong; Woo, Donghee; Kim, Suk Won; Hong, Hee-Jeon; Jo, Shin-Il; Shin, Jongheon; Oh, Ki-Bong; Oh, Dong-Chan
- Abstract
Symbiotic microorganisms associated with insects can produce a wide array of metabolic products, which provide an opportunity for the discovery of useful natural products. Selective isolation of bacterial strains associated with the dung beetle, Onthophagus lenzii , identified two strains, of which the antibiotic-producing Brevibacillus sp. PTH23 inhibited the growth of Bacillus sp. CCARM 9248, which is most closely related to the well-known entomopathogen, Bacillus thuringiensis. A comprehensive chemical investigation based on antibiotic activity discovered two new antibiotics, named lenzimycins A and B (1 - 2), which inhibited growth of Bacillus sp. CCARM 9248. The 1H and 13C NMR, MS, MS/MS, and IR analyses elucidated the structures of 1 and 2 , which comprised a novel combination of fatty acid (12-methyltetradecanoic acid), glycerol, sulfate, and N -methyl ethanolamine. Furthermore, the acid hydrolysis of 1 revealed the absolute configuration of 12-methyltetradecanoic acid as 12 S by comparing its optical rotation value with authentic (R)- and (S)-12-methyltetradecanoic acid. In addition to inhibition of Bacillus sp. CCARM 9248, lenzimycins A and B were found to inhibit the growth of some human pathogenic bacteria, including Enterococcus faecium and certain strains of Enterococcus faecalis. Furthermore, the present study elucidated that lenzimycins A and B activated a reporter system designed to detect the bacterial cell envelope stress, thereby indicating an activity against the integrity of the bacterial cell wall.
- Subjects
DUNG beetles; BACTERIAL cell walls; ENTEROCOCCUS; ENTEROCOCCUS faecium; LINEZOLID; METABOLITES; OPTICAL rotation; ETHANOLAMINES
- Publication
Frontiers in Microbiology, 2020, Vol 11, pN.PAG
- ISSN
1664-302X
- Publication type
Article
- DOI
10.3389/fmicb.2020.599911