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- Title
Screening of at‐risk blood donors for Chagas disease in non‐endemic countries: Lessons from a 2‐year experience in Tuscany, Italy.
- Authors
Mangano, Valentina D.; Prato, Marco; Marvelli, Antonella; Moscato, Giovanna; Bruschi, Fabrizio
- Abstract
Background: Chagas disease (CD) is caused by the protozoan parasite Trypanosoma cruzi and is transmitted by blood‐sucking triatomine insects in endemic areas of Latin America. Transmission can also occur via blood transfusion and is a major cause of CD in non‐endemic areas. Objectives: The aim of the study was to assess the prevalence of anti‐T. cruzi antibodies in blood donors at risk of infection in Tuscany, Italy, following the introduction of blood safety Italian legislation. Material and methods: Donors (N = 1985) were tested in 2016 to 2018 for anti‐T. cruzi IgG using an immunochromatographic test (ICT). Chemiluminescent immunoassay (CLIA) was performed on ICT‐positive donors to exclude CD, whereas enzyme‐linked immunosorbent assay and western blot were performed in case of discordant results. All assays were performed on CD patients (N = 10) for validation. Results: Ten blood donors had a positive ICT result, with a resulting T. cruzi seroprevalence of 0.5% but demonstrated negative results to CLIA, as well as to the other serological assays. The comparison of serological assays suggested a lower relative sensitivity of ICT. Conclusion: The results of this study confirm the significance of serological testing in the screening strategy for CD. However, they provide evidence for discontinuing the use of ICT as a screening test and suggest that a sensitive, specific and multi‐sample format assay should be used at the national level for uniformity of results.
- Subjects
TUSCANY (Italy); CHAGAS' disease; BLOOD donors; ENZYME-linked immunosorbent assay; BLOODSUCKING insects; SERODIAGNOSIS; TRICHOMONIASIS
- Publication
Transfusion Medicine, 2021, Vol 31, Issue 1, p63
- ISSN
0958-7578
- Publication type
Article
- DOI
10.1111/tme.12741