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- Title
Prediction of relapse by day 100 BCR-ABL quantification after allogeneic stem cell transplantation for chronic myeloid leukemia.
- Authors
Asnafi, V.; Rubio, M.-T.; Delabesse, E.; Villar, E.; Davi, F.; Damaj, G.; Hirsch, I.; Dhédin, N.; Vernant, J. P.; Varet, B.; Buzyn, A.; Macintyre, E.
- Abstract
Chronic myeloid leukemia (CML) relapse after allogeneic stem cell transplantation (SCT) is a relatively frequent situation, which is correlated to disease status, time from diagnosis to transplant and T-cell depletion. We evaluated the potential for early minimal residual disease (MRD) BCR-ABL quantification to predict relapse of CML patients receiving allogeneic SCT. Minimal residual disease was analyzed by real-time quantitative reverse transcriptase-polymerase chain reaction (RQ-PCR) at day 100 (d100) in 38 patients with >1 year follow-up after conventional non-T-cell-depleted SCT. Normal ABL control values from 1724 follow-up blood samples were used to define an RQ-PCR amplifiability index and the limits of reliable use of BCR-ABL ratios. We then compared the 14 patients with a high-level d100 BCR-ABL/ABL ratio (10−4) to that of the 24 patients with a negative/low-level ratio (<10−4). Despite being comparable for all classical parameters, the incidence of relapse was significantly higher in the high MRD group (11/14 (79%)) compared to that of the low/negative MRD group (7/24 (29%)) (P=0.009), with d100 MRD values representing an independent risk factor of relapse and disease-free survival, but not of overall survival, in multivariate analysis. These data should facilitate risk-adapted post-transplant immunosuppression and/or tyrosine kinase inhibitor therapy based on an early evaluation of MRD.Leukemia (2006) 20, 793–799. doi:10.1038/sj.leu.2404170; published online 16 March 2006
- Subjects
CHRONIC myeloid leukemia; STEM cell transplantation; MYELOID leukemia; T cells; CANCER relapse
- Publication
Leukemia (08876924), 2006, Vol 20, Issue 5, p793
- ISSN
0887-6924
- Publication type
Article
- DOI
10.1038/sj.leu.2404170