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- Title
Centrosome aberrations as a possible mechanism for chromosomal instability in non-Hodgkin's lymphoma.
- Authors
Krämer, A.; Schweizer, S.; Neben, K.; Giesecke, C.; Kalla, J.; Katzenberger, T.; Benner, A.; Müller-Hermelink, H. K.; Ho, A. D.; Ott, G.
- Abstract
Recently, centrosome aberrations have been described as a possible cause of aneuploidy in many solid tumors. To investigate whether centrosome aberrations occur in non-Hodgkin's lymphoma (NHL) and correlate with histologic subtype, karyotype, and other biological disease features, we examined 24 follicular lymphomas (FL), 18 diffuse large-B-cell lymphomas (DLCL), 33 mantle cell lymphomas (MCL), and 17 extranodal marginal zone B-cell lymphomas (MZBCL), using antibodies to centrosomal proteins. All 92 NHL displayed numerical and structural centrosome aberrations as compared to nonmalignant lymphoid tissue. Centrosome abnormalities were detectable in 32.3% of the cells in NHL, but in only 5.5% of lymphoid cells from 30 control individuals (P<0.0001). Indolent FL and MZBCL contained only 25.8 and 28.8% cells with abnormal centrosomes. In contrast, aggressive DLCL and MCL harbored centrosome aberrations in 41.8 and 35.0% of the cells, respectively (P<0.0001). Centrosomal aberrations correlated to lymphoma grade, mitotic, and proliferation indices, but not to the p53 labeling index. Importantly, diploid MCL contained 31.2% cells with abnormal centrosomes, while tetraploid samples harbored centrosome aberrations in 55.6% of the cells (P<0.0001). These results indicate that centrosome defects are common in NHL and suggest that they may contribute to the acquisition of chromosomal instability typically seen in NHL.Leukemia (2003) 17, 2207-2213. doi:10.1038/sj.leu.2403142 Published online 18 September 2003
- Subjects
CENTROSOMES; ANEUPLOIDY; TUMORS; KARYOTYPES; LYMPHOMAS; B cells; LYMPHOID tissue
- Publication
Leukemia (08876924), 2003, Vol 17, Issue 11, p2207
- ISSN
0887-6924
- Publication type
Article
- DOI
10.1038/sj.leu.2403142