We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
RAPID DETECTION OF LYMPHOMA-SPECIFIC TRANSLOCATIONS IN INTERPHASE NUCLEI OF NON-HODGKIN'S LYMPHOMA BY FLUORESCENCE IN SITU HYBRIDIZATION.
- Authors
TANIWAKI, Masafumi; UEDA, Yutaka; NISHIDA, Kazuhiro; TAKASHIMA, Teruyuki; KASHIMA, Kei; MATSUDA, Fumihiko; SILVERMAN, Gary A.
- Abstract
We have recently developed a method to detect tumor-specific rearrangement of the IgH gene in interphase nuclei by fluorescence in situ hybridization. Tumor-specific IgH gene rearrangement is equivalent to 14q32.33 translocation. Using this approach, we detected 14q32.33 translocation in 29 of 70 patients with B-cell non-Hodgkin's lymphoma (NHL). Chromosome t(3; 14) was found in 10 of these 29 patients, and were demonstrated as a fusion signal of BCL6 and VH gone probes in interphase nuclei. Furthermore, in another series of 11 patients and a NHL cell line, we demonstrated t(14;18) and t(11;14) in interphase and metaphase cells with a combination of BCL2 (or PRAD1) with IgH gone probes. Interphase FISH with lymphoma-associated gene probes is a rapid procedure for cytogenetic diagnosis of B-cell NHL.
- Subjects
CHROMOSOMAL translocation; LYMPHOMA diagnosis; DIAGNOSTIC use of fluorescence in situ hybridization; IMMUNOGLOBULIN genes; B cells; DNA probes
- Publication
Leukemia (08876924), 1997, Vol 11, p291
- ISSN
0887-6924
- Publication type
Conference Paper