We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Relationship between the A(8002)G intronic polymorphism of pre-pro-endothelin-1 gene and the endothelin-1 concentration among Tunisian coronary patients.
- Authors
Chalghoum, Abdelkader; Noichri, Yosri; Dandana, Azza; Azaiez, Sana; Baudin, Bruno; Jeridi, Gouider; Miled, Abdelhédi; Ferchichi, Salima
- Abstract
<bold>Background: </bold>Acute coronary syndromes (ACS) are complex and polygenic diseases which are a real problem of public health. These syndromes require multidisciplinary studies to understand the pathogenesis mechanisms. Our study aims to evaluate the endothelin-1 (ET-1) serum concentration in Tunisian coronary compared to controls healthy, as well as the study of the impact of an intronic polymorphism A (8002) G of pre-pro-endothelin-1 Gene (inactive precursor of ET-1) on the change in serum endothelin-1 and in the susceptibility to Acute coronary syndrome (SCA).<bold>Methods: </bold>Our samples were subdivided into coronary patients (157) and healthy subjects (142). The quantification of the ET-1 concentration was performed by high performance liquid chromatography, the identification of the different genotypes of the polymorphism A(8002)G was made by PCR-RFLP. The association between the ET-1 concentration and identified genotypes was realized by adapted software for descriptive statistics, Statistical Package for the Sociological Sciences (SPSS v 21.0).<bold>Results: </bold>Our study showed that the concentration of ET-1 was significantly higher in patients compared to controls and that the mutated allele prevails in patients F (G) = 0.78 and there is a minority in controls F (G) = 0.3. Secondly the homozygous genotype GG is associated with higher concentrations of ET-1 in patients and controls, heterozygous genotype AG is associated with intermediaries' values and AA genotype is related to lower values.<bold>Conclusion: </bold>Although the polymorphism studied is an intronic polymorphism, it is involved in the change in serum concentration of ET-1 and is a candidate gene in susceptibility to SCA. Cardiovascular diseases are "polygenic" pathology and do not obey of the law for transmission of Mendel.
- Subjects
TUNISIA; ACUTE coronary syndrome; MONOGENIC &; polygenic inheritance (Genetics); ENDOTHELINS; TUNISIANS; LIQUID chromatography; MEDICAL care; BIOCHEMISTRY; DISEASE susceptibility; GENES; GENETIC polymorphisms; GENETIC techniques; PHENOMENOLOGY; RISK assessment; PHENOTYPES; GENETIC markers; RETROSPECTIVE studies; GENETIC carriers; GENOTYPES; DIAGNOSIS
- Publication
BMC Cardiovascular Disorders, 2015, Vol 15, Issue 1, p1
- ISSN
1471-2261
- Publication type
journal article
- DOI
10.1186/s12872-015-0142-x