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- Title
Gentamicin incorporation along the nephron: Autoradiographie study on isolated tubules.
- Authors
Vandewalle, Alain; Farman, Nicolette; Morin, Jean-Paul; Fillastre, Jean-Paul; Hatt, Pierre-Yves; Bonvalet, Jean-Paul; Gastineau, M.; Wanstok, F.
- Abstract
Gentamicin is a nephrotoxic antibiotic of the aminoglycoside group, which accumulates within the renal cortex. The present study is an attempt to localize precisely the sites of gentamicin accumulation along isolated tubular segments. We performed autoradiography of ³H-gentamicin (³H-G) uptake on isolated tubules from kidneys of 6 rabbits previously treated by a single dose of this drug (125 μCi/kg of body wt; 140 μg/kg of body wt). Isolated tubules were obtained by microdissection following collagenase incubation, 4 hours after ³H-G administration. Autoradiography of single isolated tubular segments was performed according to a dry-film technique. Results were as follows. Almost no gentamicin incorporation (< 2 silver grains per 150 μm²) takes place along the distal parts of the nephron, from the beginning of the loop of Henle to the end of the medullary collecting duct. No differences were visible along these parts of the nephron, whatever their localization, conical or medullary. In the proximal tubule (PT), we observed a gradual regular increase in ³H-G accumulation, from the glomerulus to the end of the pars recta. The silver grain density progressively increased along this structure from the very early PT (5 per 150 μm²) to the last millimeter of the pars recta (40 per 150 μm²). No clear difference between superficial and juxtamedullary nephrons was detected. The possible mechanisms that could account for this observed variation in ³H-G cellular uptake along the PT are discussed.
- Subjects
GENTAMICIN; KIDNEY tubules; AUTORADIOGRAPHY; ANTIBACTERIAL agents; BILE ducts; KIDNEY glomerulus
- Publication
Kidney International, 1981, Vol 19, Issue 4, p529
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1038/ki.1981.50