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- Title
Determining the optimal protocol for preparing an acellular scaffold of tissue engineered small-diameter blood vessels.
- Authors
Pu, Lei; Wu, Jian; Pan, Xingna; Hou, Zongliu; Zhang, Jing; Chen, Wenmin; Na, Zhuhui; Meng, Mingyao; Ni, Haiyan; Wang, Liqiong; Li, Yaxiong; Jiang, Lihong
- Abstract
Although detergent-based decellularization protocols have been widely used to obtain a natural extracellular matrix (ECM) scaffold in tissue engineering, some key challenges still exist. To achieve an optimum natural decellularized scaffold for the construction of tissue-engineered small-diameter blood vessels (TEBV), porcine carotid arteries (PCAs) were decellularized by combining sodium dodecyl sulfate (SDS), sodium deoxycholate (SDC) and Triton X-100 (Triton) in different concentrations. Tissue samples were processed and their histological, biochemical and biomechanical characteristics were investigated. Results showed that only two methods 0.5% (SDS + SDC) and 1% (SDS + SDC) could completely remove of the cellular contents and preserve the native ECM architecture. Furthermore, 1% (SDS + SDC) based methods acquire preferable porosity and suitable mechanical strength. Residual Triton in the ECM scaffold holds intensive cytotoxity. In conclusion, 1%(SDS + SDC) based method can obtain a superior PCAs scaffold for the construction of TEBV. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 619-631, 2018.
- Publication
Journal of Biomedical Materials Research, Part B: Applied Biomaterials, 2018, Vol 106, Issue 2, p619
- ISSN
1552-4973
- Publication type
Article
- DOI
10.1002/jbm.b.33827