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- Title
Characterization of a new serotype g isolate of Aggregatibacter actinomycetemcomitans.
- Authors
Takada, K.; Saito, M.; Tsuzukibashi, O.; Kawashima, Y.; Ishida, S.; Hirasawa, M.
- Abstract
Aggregatibacter actinomycetemcomitans is usually isolated from the oral cavity where it is associated with active periodontitis. The species can be divided into six serotypes (a–f) according to their surface carbohydrate antigens. However, some clinical isolates cannot be grouped within these six serotypes. Gram-negative, facultative anaerobic, catalase-positive coccobacilli were isolated from a patient with periodontitis and identified by employing genetic, biochemical and serological analyses. Phenotypic data identified the isolate as A. actinomycetemcomitans. Serotype-specific polysaccharide antigen from the isolate was untypeable by immunodiffusion testing in comparison with reference A. actinomycetemcomitans serotype a to f strains. Biofilm formation by the isolate was strong but cytotoxic activity was low. Gas chromatography/mass spectroscopy analysis of partially methylated alditol acetates from surface polysaccharide showed the presence of 2,4-di- O-methyl-rhamnose and 2,3,6-tri- O-methyl-glucose, with a 1 : 1 m ratio. The 1H- and 13C-nuclear magnetic resonance spectra of the antigen showed that both constituent glycoses had α-anomeric configuration. It is proposed that the untyped strain is a new A. actinomycetemcomitans serotype, designated serotype g.
- Subjects
SEROTYPES; ACTINOMYCES; DENTAL caries; PERIODONTITIS; ANTIGENS; CARBOHYDRATES; ANAEROBIC bacteria; CATALASE; BIOCHEMISTRY
- Publication
Molecular Oral Microbiology, 2010, Vol 25, Issue 3, p200
- ISSN
2041-1006
- Publication type
Article
- DOI
10.1111/j.2041-1014.2010.00572.x