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- Title
Carboxyl terminal activating region 3 of latent membrane protein 1 encoded by the Epstein-Barr virus regulates cell proliferation and protein expression in NP69 cells.
- Authors
Zhang, Zhi-Wei; Zhang, He-Liang; Yu, Yan-Hui; Ouyang, Yong-Mei; Chen, Zhu-Chu; He, Xiu-Sheng; He, Zhi-Min
- Abstract
In the present study, the mechanism by which carboxyl terminal activating region 3 (CTAR3) of latent membrane protein 1 (LMP1), encoded by the Epstein-Barr virus, regulated cell proliferation and protein expression was investigated in the nasopharyngeal epithelial cell line NP69. The deletion mutant LMP1 (LMP1Δ232-351; amino acid residues including 232–351 codons in CTAR3 deleted) was generated by polymerase chain reaction. An NP69-LMP1Δ232-351 cell line was established by retroviral infection. Finally, cell proliferation and protein expression of NP69 cells expressing LMP1Δ232-351 were examined using a cell growth curve and western blot analysis. The results demonstrated: i) The proliferation of NP69-LMP1Δ232-351 cells was significantly decreased compared with cells expressing wild type LMP1 (LMP1WT; n=3; P<0.05); ii) 17 proteins exhibited differential protein expression (>2-fold change) in NP69-LMP1Δ232-351 cells compared with NP69-LMP1WT cells; and iii) LMP1WT was involved in activating the Janus kinase 3 (JAK3) promoter and regulating the expression of JAK3 protein, while LMP1Δ232-351 was almost defective in ability to activate the JAK promoter. These results suggested that LMP1-CTAR3 may be an important functional domain for regulating cell proliferation and protein expression in nasopharyngeal epithelial cells.
- Subjects
PROTEIN expression; MEMBRANE proteins; CELL proliferation; EPSTEIN-Barr virus; AMINO acid residues
- Publication
Molecular Medicine Reports, 2020, Vol 21, Issue 2, p720
- ISSN
1791-2997
- Publication type
Article
- DOI
10.3892/mmr.2019.10859