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- Title
Expression, purification and epitope analysis of Pla a 2 allergen from Platanus acerifolia pollen.
- Authors
Wang, De-Wang; Ni, Wei-Wei; Zhou, Yan-Jun; Huang, Wen; Cao, Meng-Da; Meng, Ling; Wei, Ji-Fu
- Abstract
Platanus acerifolia is one of the major sources of outdoor allergens to humans, and can induce allergic asthma, rhinitis, dermatitis and other allergic diseases. Pla a 2 is a polygalacturonase and represents the major allergen identified in P. acerifolia pollen. The aim of the present study was to express and purify Pla a 2, and to predict B and T cell epitopes of Pla a 2. The gene encoding Pla a 2 was cloned into the pET28a vector and subsequently transfected into ArcticExpress™ (DE3) Escherichia coli cells; purified Pla a 2 was analyzed by western blot analysis. The results of the present study revealed that the Pla a 2 allergen has the ability to bind immunoglobulin E within the sera of patients allergic to P. acerifolia pollen. In addition, the B cell epitopes of Pla a 2 were predicted using the DNAStar Protean system, Bioinformatics Predicted Antigenic Peptides and BepiPred 1.0 software; T cell epitopes were predicted using NetMHCIIpan -3.0 and -2.2. In total, eight B cell epitopes (15-24, 60-66, 78-86, 109-124, 232-240, 260-269, 298-306 and 315-322) and five T cell epitopes (62-67, 86-91, 125-132, 217-222 and 343-350) were predicted in the present study. These findings may be used to improve allergen immunotherapies and reduce the frequency of pollen-associated allergic reactions.
- Subjects
IMMUNOGLOBULIN E; IMMUNOTHERAPY; IMMUNOLOGIC diseases; ALLERGY prevention; HOUSE dust mites
- Publication
Molecular Medicine Reports, 2018, Vol 17, Issue 1, p394
- ISSN
1791-2997
- Publication type
Article
- DOI
10.3892/mmr.2017.7883