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- Title
INACTIVATION OFESCHERICHIA COLIO157:H7 DURING THE STORAGE UNDER REFRIGERATION OF APPLE JUICE TREATED BY PULSED ELECTRIC FIELDS.
- Authors
GARCÍA, DIEGO; HASSANI, MOUNIR; MAÑAS, PILAR; CONDÓN, SANTIAGO; PAGÁN, RAFAEL
- Abstract
The sensitivity of pulsed electric fields (PEF)-treatedE. coliO157:H7 cells to subsequent holding in apple juice has been evaluated.Escherichia coliO157:H7 cells in apple juice were resistant to PEF. A PEF treatment of 400 µs at any electrical field strength was not sufficient to inactivate one log 10 cycle of cells. However, PEF injured a large proportion ofE. coliO157:H7 cells that became sensitive to a subsequent storage under refrigeration in apple juice. The total lethal effect of the combined process depended on the electrical field strength and storage time. The combination of a PEF treatment at 25 kV/cm for 400 µs and a subsequent storage of the apple juice under refrigeration for 48 h allowed five log 10 cycles of inactivation to be achieved. The combination of PEF and maintenance under refrigeration has been demonstrated to be an effective pasteurization method, by sufficiently reducing the presence ofE. coliO157:H7 in apple juice in order to meet U.S. FDA recommendations.
- Subjects
ESCHERICHIA coli; ELECTRIC fields; APPLE juice; FRUIT juices; CELLS
- Publication
Journal of Food Safety, 2005, Vol 25, Issue 1, p30
- ISSN
0149-6085
- Publication type
Article
- DOI
10.1111/j.0149-6085.2005.25552.x