We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Embryo selection strategy in sibling oocytes: A novel approach to measuring the likelihood of single?embryo transfer using a mouse embryo transfer model.
- Authors
FENG-QIN XU; RONG LIU; YING MA; LI LIU; JU-YAN LIANG; YAN MA
- Abstract
The present study investigated the effect of embryo transfer on post-implantation development using different fertilization approaches in sibling oocyte procedures. C57BL/6, DBA/2, C3H/HeJ and ICR mice were used at 8-10 weeks of age. Mature oocytes were collected, divided into two groups and fertilized using in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). Different numbers of the resulting blastocysts were then transferred into recipients and designated as either: The single embryo transfer (SET) I model (one transferred embryo), the SET II model (six transferred embryos) or the multiple embryo transfer (MET) model (24 transferred embryos). The development efficiency, cell number, number of apoptotic cells in blastocysts, pregnancy efficiency, delivery rate and cumulative pregnancy efficiency were analyzed. IVF-fertilized embryos exhibited higher blas-tocyst development competence and embryo quality compared with ICSI embryos. The pregnancy and delivery efficiency was not identified to be significantly different between the two SET models, but it was lower in these two models than in the MET model. The cumulative pregnancy efficiency in SET models, calculated using a mathematical equation, was not decreased. In conclusion, embryo quality was shown to be the primary factor in selecting embryos prior to embryo transfer using sibling oocytes. In addition, single blastocyst transfer can be performed in sibling oocytes without compromising cumulative pregnancy rates, independent of the fertilization approach.
- Subjects
EMBRYO transfer; INTRACYTOPLASMIC sperm injection; FERTILIZATION in vitro; PREGNANCY; ANIMAL models in research
- Publication
Molecular Medicine Reports, 2014, Vol 9, Issue 5, p1952
- ISSN
1791-2997
- Publication type
Article
- DOI
10.3892/mmr.2014.1998