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- Title
Applications of diagonal chromatography for proteome-wide characterization of protein modifications and activity-based analyses.
- Authors
Gevaert, Kris; Impens, Francis; Van Damme, Petra; Ghesquière, Bart; Hanoulle, Xavier; Vandekerckhove, Joël
- Abstract
Numerous gel-free proteomics techniques have been reported over the past few years, introducing a move from proteins to peptides as bits of information in qualitative and quantitative proteome studies. Many shotgun proteomics techniques randomly sample thousands of peptides in a qualitative and quantitative manner but overlook the vast majority of protein modifications that are often crucial for proper protein structure and function. Peptide-based proteomic approaches have thus been developed to profile a diverse set of modifications including, but not at all limited, to phosphorylation, glycosylation and ubiquitination. Typical here is that each modification needs a specific, tailor-made analytical procedure. In this minireview, we discuss how one technique − diagonal reverse-phase chromatography − is applied to study two different types of protein modification: protein processing and protein N-glycosylation. Additionally, we discuss an activity-based proteome study in which purine-binding proteins were profiled by diagonal chromatography.
- Subjects
CHROMATOGRAPHIC analysis; PROTEINS; MODIFICATIONS; CARRIER proteins; PEPTIDES; PHOSPHORYLATION; GLYCOSYLATION
- Publication
FEBS Journal, 2007, Vol 274, Issue 24, p6277
- ISSN
1742-464X
- Publication type
Article
- DOI
10.1111/j.1742-4658.2007.06149.x