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- Title
Infection of Brain Organoids and 2D Cortical Neurons with SARS-CoV-2 Pseudovirus.
- Authors
Yi, Sang Ah; Nam, Ki Hong; Yun, Jihye; Gim, Dongmin; Joe, Daeho; Kim, Yong Ho; Kim, Han-Joo; Han, Jeung-Whan; Lee, Jaecheol
- Abstract
Since the global outbreak of SARS-CoV-2 (COVID-19), infections of diverse human organs along with multiple symptoms continue to be reported. However, the susceptibility of the brain to SARS-CoV-2, and the mechanisms underlying neurological infection are still elusive. Here, we utilized human embryonic stem cell-derived brain organoids and monolayer cortical neurons to investigate infection of brain with pseudotyped SARS-CoV-2 viral particles. Spike-containing SARS-CoV-2 pseudovirus infected neural layers within brain organoids. The expression of ACE2, a host cell receptor for SARS-CoV-2, was sustained during the development of brain organoids, especially in the somas of mature neurons, while remaining rare in neural stem cells. However, pseudotyped SARS-CoV-2 was observed in the axon of neurons, which lack ACE2. Neural infectivity of SARS-CoV-2 pseudovirus did not increase in proportion to viral load, but only 10% of neurons were infected. Our findings demonstrate that brain organoids provide a useful model for investigating SARS-CoV-2 entry into the human brain and elucidating the susceptibility of the brain to SARS-CoV-2.
- Subjects
SARS-CoV-2; NEURAL stem cells; NEURONS; COVID-19; ORGANOIDS; EMBRYONIC stem cells; CELL receptors; VIRAL load
- Publication
Viruses (1999-4915), 2020, Vol 12, Issue 9, p1004
- ISSN
1999-4915
- Publication type
Article
- DOI
10.3390/v12091004