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- Title
Quantification of AlO nanoparticles in human cell lines applying inductively coupled plasma mass spectrometry (neb-ICP-MS, LA-ICP-MS) and flow cytometry-based methods.
- Authors
Böhme, Steffi; Stärk, Hans-Joachim; Meißner, Tobias; Springer, Armin; Reemtsma, Thorsten; Kühnel, Dana; Busch, Wibke
- Abstract
In order to quantify and compare the uptake of aluminum oxide nanoparticles of three different sizes into two human cell lines (skin keratinocytes (HaCaT) and lung epithelial cells (A549)), three analytical methods were applied: digestion followed by nebulization inductively coupled plasma mass spectrometry (neb-ICP-MS), direct laser ablation ICP-MS (LA-ICP-MS), and flow cytometry. Light and electron microscopy revealed an accumulation and agglomeration of all particle types within the cell cytoplasm, whereas no particles were detected in the cell nuclei. The internalized AlO particles exerted no toxicity in the two cell lines after 24 h of exposure. The smallest particles with a primary particle size ( x) of 14 nm (Alu1) showed the lowest sedimentation velocity within the cell culture media, but were calculated to have settled completely after 20 h. Alu2 ( x = 111 nm) and Alu3 ( x = 750 nm) were calculated to reach the cell surface after 7 h and 3 min, respectively. The internal concentrations determined with the different methods lay in a comparable range of 2-8 µg AlO/cm cell layer, indicating the suitability of all methods to quantify the nanoparticle uptake. Nevertheless, particle size limitations of analytical methods using optical devices were demonstrated for LA-ICP-MS and flow cytometry. Furthermore, the consideration and comparison of particle properties as parameters for particle internalization revealed the particle size and the exposure concentration as determining factors for particle uptake.
- Subjects
ALUMINUM oxide; METAL nanoparticles; CELL lines; INDUCTIVELY coupled plasma mass spectrometry; FLOW cytometry; PARTICLE size distribution; KERATINOCYTES; EPITHELIAL cells
- Publication
Journal of Nanoparticle Research, 2014, Vol 16, Issue 9, p1
- ISSN
1388-0764
- Publication type
Article
- DOI
10.1007/s11051-014-2592-y