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- Title
Domain motions of class I release factor induced by binding with class II release factor from Euplotes octocarinatus.
- Authors
Chen, Jie; Yang, Bing-sheng; Liang, Ai-hua
- Abstract
The binding of both factors (eRF1 and eRF3) is essential for fast kinetics of the termination of protein translation. The C-terminal domain of eRF1 is known to interact with the C domain of eRF3. Eo-eRF1b contains two highly conserved tryptophan residues (W-11 and W-373), W-11 located in the Eo-eRF1b N domain and W-373 located in the EoeRF1b C domain. Fluorimetry was used to study the interactions of the proteins. When binding with Eo-eRF3Cm6, the emission peak of Eo-eRF1b is blue shifted, while the emission peak of Eo-eRF1bC has no notable change. Our results suggest that the eRF1-eRF3 interaction induces the N and C domain of eRF1b to become closer to each other.
- Subjects
PROTEINS; EUPLOTES octocarinatus; TRYPTOPHAN; FLUORIMETRY; FLUORESCENCE quenching; BIOMOLECULES
- Publication
Biochemistry (00062979), 2012, Vol 77, Issue 8, p896
- ISSN
0006-2979
- Publication type
Article
- DOI
10.1134/S000629791208010X