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- Title
Exogenous ciliary neurotrophic factor (CNTF) reduces synaptic depression during repetitive stimulation.
- Authors
Garcia, Neus; Santafé, Manel M.; Tomàs, Marta; Priego, Mercedes; Obis, Teresa; Lanuza, Maria A.; Besalduch, Nuria; Tomàs, Josep
- Abstract
It has been shown that ciliary neurotrophic factor (CNTF) has trophic and maintenance effects on several types of peripheral and central neurons, glia, and cells outside the nervous system. Both CNTF and its receptor, CNTF-R α, are expressed in the muscle. We use confocal immunocytochemistry to show that the trophic cytokine and its receptor are present in the pre- and post-synaptic sites of the neuromuscular junctions (NMJs). Applied CNTF (7.5-200 ng/ml, 60 min-3 h) does not acutely affect spontaneous potentials (size or frequency) or quantal content of the evoked acetylcholine release from post-natal (in weak or strong axonal inputs on dually innervated end plates or in the most mature singly innervated synapses at P6) or adult (P30) NMJ of Levator auris longus muscle of the mice. However, CNTF reduces roughly 50% the depression produced by repetitive stimulation (40 Hz, 2 min) on the adult NMJs. Our findings indicate that, unlike neurotrophins, exogenous CNTF does not acutely modulate transmitter release locally at the mammalian neuromuscular synapse but can protect mature end plates from activity-induced synaptic depression.
- Subjects
MYONEURAL junction; ANIMAL experimentation; BIOLOGICAL assay; CELL culture; CELLULAR signal transduction; CYTOCHEMISTRY; ELECTROPHYSIOLOGY; IMMUNOHISTOCHEMISTRY; MICE; NEUROTRANSMITTERS; RESEARCH funding; WESTERN immunoblotting; DATA analysis software; CILIARY neurotrophic factor; DESCRIPTIVE statistics; PHYSIOLOGY
- Publication
Journal of the Peripheral Nervous System, 2012, Vol 17, Issue 3, p312
- ISSN
1085-9489
- Publication type
Article
- DOI
10.1111/j.1529-8027.2012.00419.x