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- Title
In vitro culture and acclimatization of Cattleya xanthina (Orchidaceae), an endangered orchid of the Brazilian Atlantic Rainforest.
- Authors
Juras, Monique Cristine R.; Jorge, Jackeline; Pescador, Rosete; de Melo Ferreira, Wagner; Tamaki, Vivian; Suzuki, Rogério M.
- Abstract
Cattleya xanthina is a Neotropical orchid endemic to the Brazilian Atlantic Rainforest, at high risk of extinction. In this paper, we investigated the effects of different culture media on C. xanthina as well as on their endogenous nitrogen status. Culture media studied: Knudson C (KC), Vacin and Went (VW), and Murashige and Skoog (MS), the latter used at two different concentration (full and half-strength; MS/2). After 180 days, plants were transferred to MS medium with different NAA and BA concentrations. In each treatment, biometric parameters were measured and the endogenous levels of photosynthetic pigments, total protein, nitrate and ammonium ions were quantified. Plants grown on KC medium had the lowest concentration of nitrogen but exhibited the greatest shoot development, production of photosynthetic pigments and total protein. Results of growth regulators showed that the highest concentration of auxin stimulated root development and the production of photosynthetic pigments, and that a higher concentration of cytokinin promoted protein synthesis and the development of shoots. Most successful acclimatization was obtained when a mixture of Sphagnum and Pinus bark was used as the substrate.
- Subjects
PLANT micropropagation; CATTLEYAS; ACCLIMATIZATION; PHOTOSYNTHETIC pigments; PLANT growing media; RAIN forests
- Publication
Rodriguésia, 2019, Vol 70, Issue 1, p1
- ISSN
0370-6583
- Publication type
Article
- DOI
10.1590/2175-7860201970014