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- Title
Optimization of cellulase-assisted extraction process and antioxidant activities of polysaccharides from Tricholomamongolicum Imai.
- Authors
Zhao, Yong‐Ming; Song, Jin‐Hui; Wang, Jin; Yang, Jian‐Ming; Wang, Zhi‐Bao; Liu, Ying‐Hui
- Abstract
BACKGROUND: Tricholoma mongolicum Imai is awell-known edible and medicinal mushroomwhich in recent years has attracted increasing attention because of its bioactivities. In this study, water-soluble polysaccharideswere extracted fromT. mongolicum Imai by cellulase-assisted extraction and their antioxidant activities were investigated. RESULTS: In order to improve the yield of polysaccharides, four variables, cellulase amount (X1), pH (X2), temperature (X3) and extraction time (X4), were investigated with a Box-Behnken design. The optimal conditions were predicted to be cellulase amount of 20 g kg-1, pH of 4.0, temperature of 50°C and extraction time of 127 min, with a predicted polysaccharide yield of 190.1 g kg-1. The actual yield of polysaccharides under these conditions was 189.6 g kg-1, which matched the predicted value well. The crude polysaccharides were purified to obtain four fractions, and characterization of each was carried out. In addition, antioxidant properties of four polysaccharides assessed by 1,1-diphenyl-2-picryldydrazyl (DPPH) and hydroxyl radical-scavenging assays indicated that polysaccharides from T. mongolicum Imai (TMIPs) possessed antioxidant activity in a dose-dependent manner. CONCLUSION: TMIPs show moderate antioxidant activities in vitro. Therefore it is suggested that TMIPs are potential natural antioxidants for use in functional foods.
- Subjects
CELLULASE biotechnology; EXTRACTION (Chemistry); ANTIOXIDANTS; POLYSACCHARIDES; FUNCTIONAL foods
- Publication
Journal of the Science of Food & Agriculture, 2016, Vol 96, Issue 13, p4484
- ISSN
0022-5142
- Publication type
Article
- DOI
10.1002/jsfa.7662