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- Title
Flexible guide-RNA design for CRISPR applications using Protospacer Workbench.
- Authors
MacPherson, Cameron Ross; Scherf, Artur
- Abstract
The article discusses the disadvantages of using the online tool, CRISPR-Cas9 endonuclease system, for single guide RNA (sgRNA) which include limited flexibility and throughput, its availability for only few organisms, and the limited mismatches on the number of target-sgRNA. The flexibility and speed provided by offline solutions such as sgRNAcas9 is stated. A chart is presented depicting the offline software, Protospacer Workbench, for rapid, flexible design of Cas9 sgRNA.
- Subjects
RNA; NUCLEIC acids; ENDONUCLEASES; GENOMICS; ENDODEOXYRIBONUCLEASES
- Publication
Nature Biotechnology, 2015, Vol 33, Issue 8, p805
- ISSN
1087-0156
- Publication type
Article
- DOI
10.1038/nbt.3291