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- Title
Kinetic study of adenosine concentrations and the expression of adenosine deaminase in mononuclear cells during hemodialysis.
- Authors
Dussol, Bertrand; Fenouillet, Emmanuel; Brunet, Phillippe; Ipurgus, Radj; Sauze, Nicole; Carrega, Louis; Mercier, Laurence; Zouher, Ibrahim; Bechis, Guy; Berland, Yvon; Guieu, Regis
- Abstract
Kinetic study of adenosine concentrations and the expression of adenosine deaminase in mononuclear cells during hemodialysis.Background.We previously showed that high intralymphocytic adenosine (Ado) concentrations are found in hemodialyzed patients due to the reduced activity of mononuclear cell adenosine deaminase (MCADA). These abnormalities contribute to the immune defect observed in HD patients. The kinetics of these abnormalities and the causes of the low MCADA activity, however, have not been investigated. Here, we addressed this question. Since interferon gamma (IFNγ) partially modulates MCADA, we also evaluated the effect of IFNγ on MCADA activity in vitro.Methods and Results.The study included 12 patients (eight men and four women) who were observed from the first to the 36th hemodialysis (HD) session, and eight healthy subjects (controls). MCADA activity and Ado concentrations were normal before the first HD session. Ado concentrations progressively increased from the first (10.5± 3.1 pmol/107 cells) to the fourth session (26.7± 3 pmol/107 cells), before stabilizing at a high level. MCADA activity increased transiently until the second session (2.2± 0.5 IU/107 cells before HD vs. 2.8± 0.6 IU/107cells), and then decreased and stabilized at a low level (1.0± 0.5 IU/107cells). The amount of MCADA mRNA transiently increased until the third session (mRNA MCADA/mRNAβ-actin: 0.6± 0.2 vs. 0.8± 0.2), and then decreased to 0.3± 0.1 at the 36th session. MCADA activity underwent a dose-dependent increase after IFNγ stimulation.Conclusion.HD affects the transcription of the gene encoding MCADA after just three HD sessions, leading to decreased MCADA activity and increased plasma concentration of Ado.
- Subjects
HEMODIALYSIS; ADENOSINES; ADENOSINE deaminase; INTERFERONS; MESSENGER RNA; BLOOD plasma
- Publication
Kidney International, 2004, Vol 66, Issue 4, p1640
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1111/j.1523-1755.2004.00930.x