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- Title
Pentoxifylline <em>in vivo</em> and <em>in vitro</em> down-regulates the expression of the intercellular adhesion molecule-1 in monocytes.
- Authors
Neuner, P.; Klosner, O.; Pourmojib, M.; Knobler, R.; Schwarz, T.
- Abstract
Since pentoxifylline (PTX) was recently recognized as a substance with antiinflammatory capacities, we studied the in vivo and in vitro effect of PTX on the expression of the intercellular adhesion molecule-1 (ICAM-1) on haman monocytes. For this purpose four healthy volunteers were treated with PTX (5 × 400mg/day) for 2 days. Monocytes were isolated before and after PTX treatment and ICAM-1 expression was investigated. As shown by fluorescence-activated cell sorter (FACS) analysis, cultured monocytes isolated after oral application of PTX expressed significantly decreased amounts of ICAM-1 when compared with monocytes collected prior to oral PTX application. Northern blot analysis revealed reduced amounts of ICAM-1 mRNA in monocytes derived from volunteers after oral PTX treatment in comparison with monocytes isolated before oral PTX administration. Similarly, in monocytes treated with PTX (200 μg/ml) in vitro ICAM-1 was found decreased both at the protein and mRNA level in comparison with untreated cells. The inhibitory effect of PTX on ICAM-1 expression in monocytes could be reversed by the addition of exogenous tumour necrosis factor-α (TNF-α; 200 U/ml) suggesting that ICAM-1 down-regulation is mediated secondary to TNF-α suppression by PTX. The specific role of TNF-α in mediating ICAM-1 expression in cultured monocytes could be confirmed by the finding that a neutralizing anti-TNF-α antibody partially down-regulated ICAM-1 expression. The observed suppressive in vivo and in vitro effects of PTX on ICAM-1 expression in monocytes may contribute to the recently described antiinflammatory effects of PTX, e.g. in sepsis or allergic contact dermatitis.
- Subjects
PENTOXIFYLLINE; ANTI-infective agents; MONOCYTES; CELL adhesion molecules; CYTOKINES; IMMUNOLOGY
- Publication
Immunology, 1997, Vol 90, Issue 3, p435
- ISSN
0019-2805
- Publication type
Article
- DOI
10.1111/j.1365-2567.1997.00435.x