We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Primary culture of neurospheres obtained from fetal mouse central nervous system and generation of inner ear hair cell immunophenotypes in vitro.
- Authors
Liu, X-W; Sun, J-W
- Abstract
Objective:To investigate whether hair cell immunophenotypes can be derived from the central nervous system.Design:We established in vitro cell cultures from embryonic day 14.5 fetal rat brain tissue, and analysed changes in the immunohistochemical features of these cell cultures following differentiation.Result:The immature neural progenitors obtained from the fetal mouse central nervous system generated cell immunophenotypes which expressed epitopes of the hair cell marker proteins myosin VIIa and Brn-3c and the supporting cell marker pan-cytokeratin.Conclusion:Neural progenitors have the potential to differentiate into inner ear hair cell and supporting cell phenotypes, and thus may be a useful material for cell transplantation therapy aiming to replace damaged inner ear hair cells.
- Subjects
CENTRAL nervous system physiology; STEM cells; HAIR cells; ANIMAL experimentation; BIOPHYSICS; CELL culture; FLUORESCENT antibody technique; IMMUNOHISTOCHEMISTRY; IMMUNOPHENOTYPING; RESEARCH methodology; MICE; NERVOUS system regeneration; PHYSIOLOGY
- Publication
Journal of Laryngology & Otology, 2011, Vol 125, Issue 7, p686
- ISSN
0022-2151
- Publication type
Article
- DOI
10.1017/S0022215110002914