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- Title
Preparation of carboxyl‐functionalized silica core‐shell microspheres and their applications in weak cation exchange chromatography, heavy metal removal, and lysozyme enrichment.
- Authors
Ahmed, Shadi Ali Hassen; Saif, Bassam; LingHui, Qian
- Abstract
Chromatography is a technique of separation based on adsorption and/or interaction of target molecules with stationary phases. Herein, we report the design and fabrication of BTDA@SiO2 core‐shell microspheres as a new class of stationary phase and demonstrate its impressive performance for chromatographic separations. The silica microspheres of BTDA@SiO2 were synthesized by in situ method with 1,3,5‐benzenetricarboxaldehyde and 3,5‐diaminobenzoic to separate peptides and proteins on high‐performance liquid chromatography. The BTDA@SiO2 core‐shell structure has a high specific surface area and retention factor of 4.27 and 8.31 for anionic and cationic peptides, respectively. The separation factor and resolution were high as well. A typical chromatogram illustrated nearly baseline resolution of the two peptides in less than 3 min. The BTDA@SiO2 was also highly stable in the pH range of 1 to 14. Furthermore, the prepared BTDA@SiO2 core‐shell material not only be used for chromatographic separation but also as heavy metal removal from water. Using a BTDA@SiO2, we also achieved a lysozyme enrichment with a maximum saturated adsorption capacity reaching 714 mg/g. In summary, BTDA@SiO2 has great application prospects and significance in separation and purification systems.
- Subjects
HEAVY metals; HIGH performance liquid chromatography; CHROMATOGRAPHIC analysis; MICROSPHERES; RF values (Chromatography); LYSOZYMES; ANGIOTENSIN I
- Publication
Journal of Separation Science, 2024, Vol 47, Issue 11, p1
- ISSN
1615-9306
- Publication type
Article
- DOI
10.1002/jssc.202400126