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- Title
Ecophysiological diversity of a novel member of the genus Alteromonas, and description of Alteromonas mediterranea sp. nov.
- Authors
Ivanova, Elena; López-Pérez, Mario; Zabalos, Mila; Nguyen, Song; Webb, Hayden; Ryan, Jason; Lagutin, Kiril; Vyssotski, Mikhail; Crawford, Russell; Rodriguez-Valera, Francisco
- Abstract
Nine non-pigmented, motile, Gram-negative bacteria originally designated as Alteromonas macleodii deep-sea ecotypes, were isolated from seawater samples collected from four separate locations; two deep-sea sites in the Mediterranean Sea and surface water of the Aegean Sea and English Channel. The six strains studied in vitro were found to tolerate up to 20 % NaCl. The DNA-DNA relatedness between the deep-sea ecotype strains was found to be between 75 and 89 %, whilst relatedness with the validly named Alteromonas species was found to be between 31 and 69 %. The average nucleotide identity (ANI) amongst the deep-sea ecotype strains was found to be 98-100 %; the in silico genome-to-genome distance (GGD), 85-100 %; the average amino acid identity (AAI) of all conserved protein-coding genes, 95-100 %; and the strains possessed 30-32 of the Karlin's genomic signature dissimilarity. The ANI between the deep-sea ecotype strains and A. macleodii ATCC 27126 and Alteromonas australica H 17 was found to be 80.6 and 74.6 %, respectively. A significant correlation was observed between the phenotypic data obtained in vitro and data retrieved in silico from whole genome sequences. The results of a phylogenetic study that incorporated a 16S rRNA gene sequence analysis, multilocus phylogenetic analysis (MLPA) and genomic analysis, together with the physiological, biochemical and chemotaxonomic data, clearly indicated that the group of deep-sea ecotype strains represents a distinct species within the genus Alteromonas. Based on these data, a new species, Alteromonas mediterranea, is proposed. The type strain is DE ( = CIP 110805 = LMG 28347 = DSM 17117).
- Subjects
MEDITERRANEAN Sea; AEGEAN Sea; ENGLISH Channel; GRAM-negative bacteria; ALTEROMONAS; NUCLEOTIDES; AMINO acids; RIBOSOMAL RNA
- Publication
Antonie van Leeuwenhoek, 2015, Vol 107, Issue 1, p119
- ISSN
0003-6072
- Publication type
Article
- DOI
10.1007/s10482-014-0309-y