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- Title
Complete genome sequence of a potyvirus infecting yam beans ( Pachyrhizus spp.) in Peru.
- Authors
Fuentes, Segundo; Heider, Bettina; Tasso, Ruby; Romero, Elisa; zum Felde, Thomas; Kreuze, Jan
- Abstract
In 2010, yam beans in a field trial in Peru showed viral disease symptoms. Graft-transmission and positive ELISA results using potyvirus-specific antibodies suggested that the symptoms could be the result of a potyviral infection. Small interfering RNA (siRNA) were extracted from one of the samples and sent for high-throughput sequencing. The full genome of a new potyvirus could be assembled from the resulting siRNA sequences, and it was sufficiently different from other sequences to be considered a member of a new species, which we have designated Yam bean mosaic virus (YBMV). Sequence similarity suggests that YBMV has also been detected in yam beans in Indonesia.
- Subjects
PERU; NUCLEOTIDE sequence; POTYVIRUS diseases; PACHYRHIZUS; ENZYME-linked immunosorbent assay; SMALL interfering RNA
- Publication
Archives of Virology, 2012, Vol 157, Issue 4, p773
- ISSN
0304-8608
- Publication type
Article
- DOI
10.1007/s00705-011-1214-6