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- Title
Immunohistochemical analysis of PDGFR-α for wound age determination.
- Authors
Zhang, Wei; Ishida, Yuko; Nosaka, Mizuho; Kuninaka, Yumi; Ishigami, Akiko; Yasuda, Haruki; Kofuna, Ayumi; Kamata, Ten; Goto, Utako; Kondo, Toshikazu
- Abstract
Immunohistochemical analysis of platelet-derived growth factor receptor-α (PDGFR-α) was performed on human skin wounds obtained from forensic autopsy cases. Thirty human skin wounds were collected at different post-infliction intervals as follows: Group I, 4 h to 3 days (n = 16); Group II, 4 to 7 days (n = 7); Group III, 9 to 10 days (n = 3); and Group IV, 14 to 20 days (n = 4). Immunopositive reactions for PDGFR-α were not observed in the uninjured human skin specimens. In a semi-quantitative morphometrical analysis, the number of PDGFR-α-positive cells was observed increased in Group II, with the average number of PDGFR-α-positive cells being the highest in Group II. Additionally, in Group II, all specimens showed PDGFR-α-positive cells, with an average number of > 200 cells in five fields of view, suggesting a wound age of 4 to 7 days. Taken together, the immunohistochemical detection of PDGFR-α in human skin wounds can be a useful tool for wound age determination.
- Subjects
IMMUNOHISTOCHEMISTRY; PLATELET-derived growth factor; SKIN injuries
- Publication
International Journal of Legal Medicine, 2024, Vol 138, Issue 4, p1351
- ISSN
0937-9827
- Publication type
Article
- DOI
10.1007/s00414-024-03208-0