We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Effect of Resveratrol on the Prevention of Intra-Abdominal Adhesion Formation in a Rat Model.
- Authors
Wei, Guangbing; Chen, Xin; Wang, Guanghui; Fan, Lin; Wang, Kang; Li, Xuqi
- Abstract
Background: Intra-abdominal adhesions are a very common complication following abdominal surgery. Our previous studies have demonstrated that the inhibition of inflammation at the sites of peritoneal injury can prevent the formation of intra-abdominal adhesions. Resveratrol is a natural extract with a broad range of anti-inflammatory effects. Therefore, we propose that resveratrol can reduce the formation of intra-abdominal adhesions after surgery. The aim of this study was to investigate the effect of resveratrol on intra-abdominal adhesion prevention in a rat model with surgery-induced peritoneal adhesions. Materials and Methods: The cecum wall and its opposite parietal peritoneum were abraded following laparotomy to induce intraabdominal adhesion formation. Varying doses of resveratrol were administered to the animals. On the eighth day after surgery, the adhesion score was assessed using a visual scoring system. Picrosirius red staining and a hydroxyproline assay were used to assess the amount of collagen deposition in the adhesion tissues. The levels of serum interleukin-6 (IL-6), tumor necrosis factor (TNF-a), and transforming growth factor beta-1 (TGF-ß1) were determined by an enzyme-linked immunosorbent assay (ELISA). Western blotting was performed to determine the protein expression of TGF-ß1, fibrinogen, and a-smooth muscle actin (a-SMA) in rat peritoneal adhesion tissue. Real-time RT-PCR was performed to quantify the mRNA expression of TGF-ß1, fibrinogen, and a-SMA. Results: Resveratrol significantly reduced intra-abdominal adhesion formation and fibrin deposition in the rat model. Furthermore, resveratrol significantly reduced the serum levels of IL-6, TNF-a, and TGF-ß1. The protein and mRNA expression of TGF-ß1, fibrinogen, and a-SMA in the rat peritoneum and adhesion tissues were also down-regulated due to resveratrol intervention. Conclusion: Resveratrol can effectively prevent the formation of postoperative intra-abdominal adhesions in a rat model. This effect may be related to the suppression of inflammatory cytokine expression in the injured peritoneum by resveratrol. This study suggests that resveratrol may be a new and effective anti-adhesive agent that is worthy of further study and has potential application value.
- Subjects
RESVERATROL; DRUG efficacy; ABDOMINAL surgery; SURGICAL complications; PERITONEUM; LABORATORY rats; WOUNDS &; injuries; THERAPEUTICS
- Publication
Cellular Physiology & Biochemistry (Karger AG), 2016, Vol 39, Issue 1, p33
- ISSN
1015-8987
- Publication type
Article
- DOI
10.1159/000445603