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- Title
Amino acid content in extruded feed mixtures.
- Authors
Trakalo, Tetiana; Shapovalenko, Oleh; Yaniuk, Tetiana
- Abstract
Introduction. To determine amino acid profile of extruded feed mixes containing flax feed extract based on water, amino acid profile amino acid profile and biological value of extruded feed mixes of different formulating composition has been studied. Materials and methods. We investigated extruded feed mixes made of wheat, maize, wheat mill-run and flax feed extract based on water (FFEBW) having different percentage. Ratio (wheat:corn:FFEBW) for mixture 1 is 40:40:20, for mixture 2 is 45:45:10. Ratio (wheat mill-run:corn:FFEBW) for mixture 3 is 40:40:20. The mixture was mixed and extruded at 110-120 °C temperature, 2-4 MPa pressure decontaminating it. The concentration of free amino acids was determined by the method of ion-exchange chromatography. Results and discussions. Undertaken researches of amino acid composition of the extruded feed mixtures allowed to identify and numerically define 9 essential amino acids (valine, leucine, isoleucine, lysin, methionine, phenylalanine, threonine, arginine, histidin) and 8 nonessential amino acids (aspartic, cystein, serine, tyrosine, glutaminic acid, glycine, pyrrolidine carboxulic acid, and alanine), calculations of amino acids score of extruded feed mixtures are also described that allows to get data on every amino acid, to define the first limited amino acid, calculate the coefficient of divergence amino acid score (CDAAS) and index of protein biological value (BV) of the investigated standards of extrudate. The most essential amino acids contains mixtures - 1 - 42,82% on 100 g of protein and - 2 - 41,27% on 100 g of protein. Content of leucine, lysin and threonine in protein of mixtures presents 8,34-9,8% 100 g, 3,44-3,84% 100 g, and 2,67-3,75% 100 g, accordingly, that confirms high protein value of extruded feed mixtures. With the help of calculations, it is determined that the greatest biological value has protein of mixture - 1 - 70,25%. This mixture appeared the most balanced among amino acid composition comparatively with other mixtures. The least index of biological value is set in mixture - 3 - 65,25%, that is explained by more considerable divergence amino acid score than other amino acids. Conclusion. It is recommended to add flax feed extracts in the complement of grain mixtures as an effective method of biological value increase of extruded feed mixtures, as hit allows to increase the content of protein and amino acids.
- Subjects
LEUCINE; AMINO acids in animal nutrition; ESSENTIAL amino acids; ANIMAL feeds; AMINO acids; MIXTURES; G proteins
- Publication
Ukrainian Journal of Food Science, 2019, Vol 7, Issue 1, p92
- ISSN
2310-1008
- Publication type
Article
- DOI
10.24263/2310-1008-2019-7-1-11