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- Title
Using of enzymes to extract of rapeseed oil by pressing.
- Authors
Cherstva, Alona; Lastovetska, Anastasia; Nosenko, Tamara
- Abstract
Introduction. To extract lipids that are stored in a locked structure seed must overcome several barriers. The use of hydrolytic enzymes such as cellulases, hemitselyulaza, pectinase increases the permeability of such structures. Materials and methods. It was used low-glucosinolate rapeseed varieties. For pre-processing enzyme selected following enzymes with extended spectrum of activity: Protolad (protease, 70 U / g enzyme, Ukraine) and Celulad (tsellyulaza, 300 U / g enzyme, Ukraine). To assess the quality of the resulting oil used methods for determining iodine, acid and peroxide numbers. Effectiveness was assessed by enzyme preparations instant shaking, and the residual amount of oil in the meal. Results and discussion. The best results were obtained in samples of rapeseed that had moisture content of 4%. Enzymatic treatment of oily material over two hours is sufficient to reduce the residual cake oil content from 24.4% to 9.4%. Number enzyme 0.4% by weight of seeds is the best value. Enzymatic treatment in compression technology is a very effective way to increase the quantitative output of oil. Physical and chemical properties of the sample oil after the enzymatic treatment compared with the sample pressed rapeseed oil. The results meet the requirements of regulatory documents. Analysis of fatty acid rapeseed oil sample after enzymatic treatment showed an increase in linoleic and oleic acid and about 3% linolenic - more than 1% compared with the control. The content of other fatty acids in rapeseed oil samples had significant differences. Calculated regression equation for the dependence of oil on the concentration of the enzyme, the moisture content in the material before pressing and hydrolysis time on the basis of experimental data. Increasing the number of removed oil from rapeseed, which is subjected to enzymatic processing prior shows that enzymatic hydrolysis is a very effective method of pretreatment material before pressing. Adding the enzyme is carried out in aqueous solution. High seed moisture during processing is an unfavorable factor in economic terms, as the next stage of processing is the drying. Conclusions. The main objectives of the study is improvement pretreatment method rapeseed, namely reducing seed moisture during enzymatic hydrolysis.
- Subjects
RAPESEED oil; PLANT enzymes; PLANT extracts
- Publication
Ukrainian Journal of Food Science, 2016, Vol 4, Issue 1, p85
- ISSN
2310-1008
- Publication type
Article