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- Title
Evaluation of Taurine by HPTLC Reveals the Mask of Adulterated Edible Bird's Nest.
- Authors
Peishan Teo; Fucui Ma; Daicheng Liu
- Abstract
Detection of amino acid is an effective and common method to determine adulteration in edible bird's nest. Therefore, a simple and sensitive method was developed to detect taurine for determining adulteration in edible bird's nest in the future. Sample was separated on precoated silica gel GF254 high-performance thin layer chromatographic plates. Separation of taurine was performed by n-propyl alcohol : ethanol : water : glacial acetic acid (5.2 : 0.8 : 2 : 2, v : v : v : v). Densitometric analysis of taurine was carried out in the absorbance mode at 485 nm. The method was validated for precision, intra- and interday variation, and recovery. This study proved that high-performance thin layer chromatography is a simple, rapid, precise, and selective method for qualitative and quantitative analysis of taurine in edible bird's nest.
- Subjects
TAURINE; THIN layer chromatography; ADULTERATIONS; EDIBLE birds' nests; ABSORBANCE scale (Spectroscopy)
- Publication
Journal of Chemistry, 2013, p1
- ISSN
2090-9063
- Publication type
Article
- DOI
10.1155/2013/325372