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- Title
Cladribine and Fludarabine Nucleoside Change the Levels of CD Antigens on B-Lymphoproliferative Disorders.
- Authors
Cassano, Carlos; Mactier, Swetlana; Mulligan, Stephen P.; Belov, Larissa; Huang, Pauline; Christopherson, Richard I.
- Abstract
The purine analogs, fludarabine nucleoside (FdA), and cladribine (CdA) (1 μM, 24 hours), significantly changed the levels of some surface antigens on the human B-cell lines MEC2 and Raji. Changes in the surface proteins were identified using a Cluster of Differentiation (CD) antibody microarray that captures live cells and confirmed by flow cytometry. For Raji cells, CdA upregulated CD10, CD54, CD80, and CD86, with repression of CD22, while FdA up-regulated CD20, CD54, CD80, CD86 and CD95. For MEC2 cells, CdA up-regulated CD11a, CD20, CD43, CD45, CD52, CD54, CD62L, CD80, CD86, and CD95, but FdA had no effect. Up-regulation of particular CD antigens induced on a B-cell lymphoproliferative disorder by a purine analog could provide targets for therapeutic antibodies with synergistic cell killing.
- Subjects
FLUDARABINE; LYMPHOPROLIFERATIVE disorders; FLOW cytometry; ANTIGENS; CELL proliferation; CELL death
- Publication
International Journal of Proteomics, 2010, p1
- ISSN
2090-2166
- Publication type
Article
- DOI
10.1155/2010/964251