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- Title
Constructing a Mouse Oct4 Promoter/EGFP Vector, as a Whole-Cellular Reporter to Monitor the Pluripotent State of Cells.
- Authors
Ghorbani, Reza; Emamzadeh, Abdolrahman; Khazaie, Yahya; Dormiani, Kianoush; Ghaedi, Kamran; Rabbani, Mohammad; Foruzanfar, Mahboubeh; Karbalaie, Khadijeh; Karamali, Fereshteh; Lachinani, Liana; Kiani-Esfahani, Abbas; Nematollahi, Marzieh; Esfahani, Mohammad Hossein Nasr
- Abstract
Background: The transcription factor Oct-4, is an important marker of undifferentiating level and a key regulating factor for maintenance of pluripotency in cells. Establishment of an Oct-4 promoter-based reporter system is an appropriate tool for monitoring the differentiation of embryonic stem cells both in vivo and in vitro. Methods: In the present study, we report construction of a recombinant vector, pDB2 Oct4 promoter/EGFP, in which expression of Enhanced Green Fluorescent Protein (EGFP) was controlled by the mouse Oct-4 promoter. Results: In transfected mouse embryonic stem cells with this vector, EGFP was predicted to be specifically expressed in pluripotency state. After transfection, high-level expression of EGFP under the control of Oct-4 promoter was observed in manipulated embryonic stem cells. Conclusion: Thus, our new cellular reporter showed that both the properties of embryonic cells and expression the EGFP could be of great help in studying the differentiating and reprogramming mechanisms of mESCs.
- Subjects
STEM cell research; BIOTECHNOLOGY methodology; ANIMAL experimentation; EPIDERMAL growth factor; GENETIC techniques; MICE; PROTEINS; RESEARCH funding; GENOMICS
- Publication
Avicenna Journal of Medical Biotechnology, 2013, Vol 5, Issue 1, p2
- ISSN
2008-2835
- Publication type
Article