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- Title
Comparison of Three Detection Methods for Seoul Virus Causing Hemorrhagic Fever with Renal Syndrome.
- Authors
Keping Chen; Tiantian Zhao; Huidi Sun; Yu Geng; Yurong Xu; Chun Shan; Yuxin Chen
- Abstract
Background: Seoul virus (SEOV) is a significant causative pathogen of hemorrhagic fever with renal syndrome (HFRS). Accurate discrimination of SEOV infection from other viral or bacterial infections holds vital clinical importance. Methods: Our study utilized quantitative real-time PCR (qRT-PCR), metagenomic next-generation sequencing (mNGS), and immunological assays to identify the pathogen causing HFRS. Results: For the case, mNGS identified SEOV and suspected host or environmental microorganisms at 5 days from symptom onset. qRT-PCR detected SEOV between 5 to 8 days from symptom onset. Anti-hantavirus IgM antibodies reached positive criteria at 7 days and IgG antibodies at 9 days from symptom onset. Conclusions: qRT-PCR, mNGS, and immunological assays each have merits and drawbacks. Optimal selection depends on laboratory conditions and clinical requirements.
- Subjects
SEOUL (Korea); HEMORRHAGIC fever with renal syndrome; BACTERIAL diseases; NUCLEOTIDE sequencing; VIRUS diseases; HANTAVIRUSES; PHOSPHOLIPID antibodies
- Publication
Clinical Laboratory, 2024, Vol 70, Issue 2, p428
- ISSN
1433-6510
- Publication type
Article
- DOI
10.7754/Clin.Lab.2023.230821