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- Title
DIVERSIFIED BIOLOGICAL ACTIVITY OF (1→3), (1→4)-α-D-GLUCAN FROM MANGO (MANGIFERA INDICA L.) FRUITS AGAINST HUMAN COLON CANCER CELLS.
- Authors
PADUCH, ROMAN; PIĘT, MATEUSZ; WLIZŁO, KAMILA; WERTEL, IWONA; PAWŁOWSKA, ANNA; TROJNAR, SYLWIA; SAJNAGA, EWA; KAZIMIERCZAK, WALDEMAR; SKOWRONEK, MARCIN; WIATER, ADRIAN
- Abstract
The aim of this research was to determine the biological activity of (1→3), (1→4)-α-D-glucan from mango (Mangifera indica L.) fruits on human colon carcinoma cells HT29 (Duke's A) and SW620 (Duke's C). Many natural substances have been described to show anti-tumor activity or limit the dissemination of transformed cells. Among them, there are polysaccharides, which are biological macromolecules consisting of different amounts of monosaccharides linked by glycosidic bonds. One of such polymers is the water-soluble (1→3), (1→4)-α-D-glucan from mango fruits. The isolated (1→3), (1→4)-α-D-glucan did not induce a significant reduction in the number of tumor cells. The viability of cells did not drop below 94% upon the use of the polymer at the concentration of 225 µg/mL. The SW620 cells were more sensitive than HT29, showing a significant decrease in the cellular metabolism after incubation with the polysaccharide at a 250 µg/mL concentration. No significant changes in the morphology of both tumor cell types after the incubation with the (1→3), (1→4)-α-D-glucan were observed. The release of nitric oxide (NOx) by the tested cells was dependent on the polysaccharide concentration. There were differences in the NOx release profile depending on the type of cells. The (1→3), (1→4)-α-D-glucan decreased the release of IL-1ß and IL-6 by both types of cells and increased the level of IL-10. Moreover, the a-D-glucan showed ferric reducing power and reduced the level of the DPPH free radical. Concluding, the analyzed (1→3), (1→4)-α-D-glucan isolated from mango fruits can limit the development of neoplastic cells not through direct cytotoxic action but via antioxidant and immunomodulatory activities.
- Subjects
GLUCANS; MANGO; COLON cancer treatment; MACROMOLECULES; POLYSACCHARIDES
- Publication
Acta Poloniae Pharmaceutica, 2022, Vol 79, Issue 3, p367
- ISSN
0001-6837
- Publication type
Article
- DOI
10.32383/appdr/152097