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- Title
Lymphoma Immunophenotype of Dogs Determined by Immunohistochemistry, Flow Cytometry, and Polymerase Chain Reaction for Antigen Receptor Rearrangements.
- Authors
Thalheim, L.; Williams, L.E.; Borst, L.B.; Fogle, J.E.; Suter, S.E.
- Abstract
Background Immunohistochemistry ( IHC), flow cytometry ( FC), and PCR for antigen receptor rearrangements ( PARR) are 3 widely utilized tests to determine immunophenotype in dogs with lymphoma ( LSA). Objectives This study evaluated the ability of FC and PARR to correctly predict immunophenotype as defined by IHC and to determine the level of agreement among the 3 tests. Animals Sixty-two dogs with lymphoma. Methods Retrospective study. Medical records were searched to identify dogs with LSA that had concurrent IHC, FC, and PARR performed. Immunophenotype results were categorized as B-cell, T-cell, dual immunophenotype (B- and T-cell), or indeterminate. The results of FC and PARR were evaluated for correctly classifying B- and T-cell LSA as compared with IHC. The sensitivity, specificity, positive predictive value ( PPV), and negative predictive value ( NPV) were evaluated in addition to concordance between each test. Results The sensitivity of FC was significantly higher than PARR for both B-cell (91% versus 67%; P < 0.0072) and T-cell (100% versus 75%; P < 0.0312) LSA. The percent agreement between FC and IHC was 94%, between PARR and IHC was 69%, between FC and PARR was 63%, and among all 3 tests was 63%. Conclusions and Clinical Importance Flow cytometry is superior to PARR in correctly predicting immunophenotype when evaluating lymph nodes from dogs already diagnosed with B- or T-cell LSA. If fresh samples are not available for FC, PARR is an acceptable assay for determination of immunophenotype given its high specificity.
- Subjects
LYMPHOMAS; HUMAN phenotype; DOG diseases; IMMUNOHISTOCHEMISTRY; FLOW cytometry; POLYMERASE chain reaction; ANTIGEN receptors
- Publication
Journal of Veterinary Internal Medicine, 2013, Vol 27, Issue 6, p1509
- ISSN
0891-6640
- Publication type
Article
- DOI
10.1111/jvim.12185