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- Title
Quantitative Detection of Horse Contamination in Cooked Meat Products by ELISA.
- Authors
THIENES, CORTLANDT P.; JONGKIT MASIRI; BENOIT, LORA A.; BARRIOS-LOPEZ, BRIANDA; SAMUEL, SANTOSH A.; COX, DAVID P.; DOBRITSA, ANATOLY P.; NADALA, CESAR; SAMADPOUR, MANSOUR
- Abstract
Concerns about the contamination of meat products with horse meat and new regulations for the declaration of meat adulterants have highlighted the need for a rapid test to detect horse meat adulteration. To address this need, Microbiologique, Inc., has developed a sandwich ELISA that can quantify the presence of horse meat down to 0.1% (w/w) in cooked pork, beef, chicken, goat, and lamb meats. This horse meat authentication ELISA has an analytical sensitivity of 0.000030 and 0.000046% (w/v) for cooked and autoclaved horse meat, respectively, and an analytical range of quantitation of 0.05-0.8% (w/v) in the absence of other meats. The assay is rapid and can be completed in 1 h and 10 min. Moreover, the assay is specific for cooked horse meat and does not demonstrate any cross-reactivity with xenogeneic cooked meat sources.
- Subjects
HORSEMEAT; MEAT contamination; FOOD inspection; FOOD safety; LAMB meat industry
- Publication
Journal of AOAC International, 2018, Vol 101, Issue 3, p817
- ISSN
1060-3271
- Publication type
Article
- DOI
10.5740/jaoacint.17-0151