We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Cardiac stem cells with electrical stimulation improve ischaemic heart function through regulation of connective tissue growth factor and miR-378.
- Authors
Kim, Sun Wook; Kim, Ha Won; Huang, Wei; Okada, Motoi; Welge, Jeffrey A.; Wang, Yigang; Ashraf, Muhammad
- Abstract
Aims In this study, we investigated whether pre-conditioning (PC) by electrical stimulation (EleS) induces cytoprotective effect on cardiac stem cells (CSCs) and determined its underlying molecular mechanisms. Methods and results Sca-1+ CSCs were isolated from male C57BL6 mice (12 weeks) hearts. PC of CSCs with EleS (EleSCSCs) was carried out for 3 h at 1.5 V followed by exposure to 300 µM H2O2 for 5 h. Cytoprotective effects and cell adhesion ability were significantly increased by EleS as evaluated by transferase-mediated dUTP nick-end labelling (TUNEL), lactate dehydrogenase (LDH) release assay, and adhesion assay. EleS increased phosphorylation of AKT, focal adhesion kinase (FAK), and glycogen synthase kinase (GSK3β), as well as decreased caspase-3 cleavage. Interestingly, inhibition of AKT or FAK abolished the pro-survival effects of EleS. We found that connective tissue growth factor (Ctgf) was responsible for EleS-induced CSC survival and adhesion.The survival rate of EleSCSCs after transplantation in the infarcted myocardium was significantly increased together with improvement in cardiac function. Importantly, knockdown of Ctgf abolished EleS-induced cytoprotective effects and recovery of cardiac function. Furthermore, we identified miR-378 as a potential Ctgf regulator in EleSCSCs. Conclusion EleS enhanced CSC survival in vitro and in vivo as well as functional recovery of the ischaemic heart through an AKT/FAK/CTGF signalling pathway. It is suggested that Ctgf and miR-378 are novel therapeutic targets for stem cell-based therapy.
- Subjects
CORONARY disease; STEM cells; PHOSPHORYLATION; CONNECTIVE tissue growth factor; NUCLEOTIDE sequence; LABORATORY mice; ELECTRIC stimulation; CELLULAR signal transduction
- Publication
Cardiovascular Research, 2013, Vol 100, Issue 2, p241
- ISSN
0008-6363
- Publication type
Article
- DOI
10.1093/cvr/cvt192