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- Title
Limitations of sandwich ELISAs for bluetongue virus antibody detection.
- Authors
Eschbaumer, M.; Schulz, C.; Wäckerlin, R.; Gauly, M.; Beer, M.; Hoffmann, B.
- Abstract
The article discusses a research study on sandwich enzyme-linked immunosorbent assay (ELISA) limitations for detecting bluetongue virus (BTV) antibody. Study subjects include 10 sheep, 12 cattle and three each of alpacas and llamas and their blood and serum samples examined after being experimentally infected with BTV serotype 8 (BTV-8) while for virus isolation, Vero cells were used. Results showed the advantages of sandwich ELISA assays such as detecting immune response and sensitivity to antibodies induced through vaccination.
- Subjects
ENZYME-linked immunosorbent assay; BLUETONGUE virus; CATTLE diseases; VETERINARY virology; VETERINARY medicine
- Publication
Veterinary Record: Journal of the British Veterinary Association, 2011, Vol 168, Issue 24, p642
- ISSN
0042-4900
- Publication type
Article
- DOI
10.1136/vr.d1184