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- Title
miR-31-5p Is a LIPUS-Mechanosensitive MicroRNA that Targets HIF-1α Signaling and Cytoskeletal Proteins.
- Authors
Costa, Viviana; Carina, Valeria; Raimondi, Lavinia; De Luca, Angela; Bellavia, Daniele; Conigliaro, Alice; Alessandro, Riccardo; Salamanna, Francesca; Fini, Milena; Giavaresi, Gianluca; Setti, Stefania
- Abstract
The roles of low-intensity pulsed ultrasound (LIPUS) and microRNAs (miRNAs) on hMSCs commitments have already been investigated; however, the effects of the application of their co-treatments in an in vitro cell model are still unknown. Our previous studies demonstrated that (i) LIPUS modulated hMSCs cytoskeletal organization and (ii) miRNA-675-5p have a role in HIF-1α signaling modulation during hMSCs osteoblast commitment. We investigated for the first time the role of LIPUS as promoter tool for miRNA expression. Thanks to bioinformatic analysis, we identified miR-31-5p as a LIPUS-induced miRNA and investigated its role through in vitro studies of gain and loss of function. Results highlighted that LIPUS stimulation induced a hypoxia adaptive cell response, which determines a reorganization of cell membrane and cytoskeleton proteins. MiR-31-5p gain and loss of function studies, demonstrated as miR-31-5p overexpression, were able to induce hypoxic and cytoskeletal responses. Moreover, the co-treatments LIPUS and miR-31-5p inhibitor abolished the hypoxic responses including angiogenesis and the expression of Rho family proteins. MiR-31-5p was identified as a LIPUS-mechanosensitive miRNAs and may be considered a new therapeutic option to promote or abolish hypoxic response and cytoskeletal organization on hMSCs during the bone regeneration process.
- Subjects
HYPOXEMIA; MESENCHYMAL stem cells; MICRORNA; RHO factor; REGENERATIVE medicine
- Publication
International Journal of Molecular Sciences, 2019, Vol 20, Issue 7, p1569
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms20071569