We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Characterization of Mast Cell Secretory Granules and Their Cell Biology.
- Authors
Azouz, Nurit Pereg; Hammel, Ilan; Sagi-Eisenberg, Ronit
- Abstract
Exocytosis and secretion of secretory granule (SG) contained inflammatory mediators is the primary mechanism by which mast cells exert their protective immune responses in host defense, as well as their pathological functions in allergic reactions and anaphylaxis. Despite their central role in mast cell function, the molecular mechanisms underlying the biogenesis and secretion of mast cell SGs remain largely unresolved. Early studies have established the lysosomal nature of the mast cell SGs and implicated SG homotypic fusion as an important step occurring during both their biogenesis and compound secretion. However, the molecular mechanisms that account for key features of this process largely remain to be defined. A novel high-resolution imaging based methodology allowed us to screen Rab GTPases for their phenotypic and functional impact and identify Rab networks that regulate mast cell secretion. This screen has identified Rab5 as a novel regulator of homotypic fusion of the mast cell SGs that thereby regulates their size and cargo composition.
- Subjects
MAST cells; EXOCYTOSIS; SECRETORY granules; CYTOLOGY; IMMUNE response; ALLERGIES; ANAPHYLAXIS
- Publication
DNA & Cell Biology, 2014, Vol 33, Issue 10, p647
- ISSN
1044-5498
- Publication type
Article
- DOI
10.1089/dna.2014.2543