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- Title
Multiplexed single-molecule assay for enzymatic activity on flow-stretched DNA.
- Authors
Sangjin Kim; Blainey, Paul C; Schroeder, Charles M; X Sunney Xie
- Abstract
We report a single-molecule assay for nucleic-acid enzymes on flow-stretched DNA templates. To facilitate the detection of slow or intermittent enzymatic activities, we developed the assay with 15-nm spatial resolution at a frame rate of 1 Hz and ∼10 nm mechanical stability over the timescale of hours. With multiplexed data collection, we applied the assay to φ29 DNA polymerase, HIV-1 reverse transcriptase, λ exonuclease and Escherichia coli RNA polymerase.
- Subjects
DNA; NUCLEIC acids; DEOXYRIBOSE; PROTEINS; BIOMOLECULES
- Publication
Nature Methods, 2007, Vol 4, Issue 5, p397
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth1037