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- Title
Akt isoforms regulate intermediate filament protein levels in epithelial carcinoma cells
- Authors
Fortier, Anne-Marie; Van Themsche, Céline; Asselin, Éric; Cadrin, Monique
- Abstract
Abstract: Keratin 8 and 18 are simple epithelial intermediate filament (IF) proteins, whose expression is differentiation- and tissue-specific, and is maintained during tumorigenesis. Vimentin IF is often co-expressed with keratins in cancer cells. Recently, IF have been proposed to be involved in signaling pathways regulating cell growth, death and motility. The PI3K/Akt pathway plays a pivotal role in these processes. Thus, we investigated the role of Akt (1 and 2) in regulating IF expression in different epithelial cancer cell lines. Over-expression of Akt1 increases K8/18 proteins. Akt2 up-regulates K18 and vimentin expression by an increased mRNA stability. To our knowledge, these results represent the first indication that Akt isoforms regulate IF expression and support the hypothesis that IFs are involved in PI3K/Akt pathway.
- Subjects
EPITHELIAL cells; CANCER cells; PROTEIN kinases; GENETIC regulation; INTERMEDIATE filament proteins; KERATIN; CARCINOGENESIS; GENE expression
- Publication
FEBS Letters, 2010, Vol 584, Issue 5, p984
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2010.01.045